UV BioSpectrum® 2D IMAGING SYSTEM

The BioSpectrum® 2D Imaging System is designed to automate 2D research with one-touch, pre-set or user-defined PC controls for accurate, repeatable imaging and analysis. Achieve fast 100% matching of 2D gels. The fully automated system design with a cooled CCD camera permits multiple applications including 2D, 1D colorimetric and chemiluminescent imaging. The Vision Works 2D software allows 100% matching of 2D spots!

APPLICATIONS

2D protein gels

Visible and fluorescent stains

Single and multiple dyes

2D western blots

 Plus 1D, colorimetric, chemiluminescent imaging

MAIN FEATURES

Capture superior images with the 8.3 megapixel high resolution, high sensitivity cooled CCD camera

Motorized optics for setting the aperture, zoom and focus

Automated templates for repeat experiments

Light tight darkroom creates optimum imaging conditions

Unique viewing window blocks UV for quick sample inspection

Built-in overhead white light and UV are easily selected from the software interface

Automated control of the five-position emission filter array

Easy access white light illuminator for imaging Coommassie blue or silver stained media

Roll-out tray provides easy access to the UV Transilluminator

Chemi tray fits over the white light plate or UV Transilluminator for placement of blots

Select from several Benchtop, patented and awarding winning Transilluminator models (configured with system or optional)

VisionWorksLS and VisionWorks 2D Software (configured with system or optional)

Accessibility ports built-in for the BioLite (fiber optic multispectral source) as an optional component

In addition to 2D imaging, the system can be used for 1D fluorescent and colorimetric imaging as well as chemiluminescent imaging

TRANSILLUMINATION

White light illuminator for visualizing samples such as Coommassie blue or silver stained media; operate the white light via the software interface

Store the white light plate in the side pocket of the BioSpectrum when not in use

To use the Transilluminator, slide the Transilluminator out on the sliding tray and place samples on the filter surface

To fluoresce samples in the 460-470mm range, place the optional Visi-Blue Plate over the Transilluminator surface

VisionWorks 2D Analysis Software

A guided workflow for straightforward, fast 2D gel analysis.

See VisionWorks 2D tab
 

Automates research for easy, yet accurate and repeatable results

Controls the motorized zoom lens, epi and transillumination UV and white light, emission filter selection and system power

Allows selection of user-defined templates or default settings

Create macros to quickly execute multiple commands with a click of a button

VisionWorksLS Image Acquisition and Analysis Software

VisionWorksLS Image Acquisition and Analysis Software can be configured with the system or ordered separately. Check with a local representative for details. This sophisticated package provides comprehensive tools for image capture and analysis of gels, plates and membranes as well as colony counting. Capabilities include:

Image acquisition functions for simple image capture and expanded integration options

Apply numerous image enhancements to captured images

Comprehensive tools for advanced 1D lane densitometry and analysis including area density, molecular weights and colony counting

Support for 21 CFR Part 11 compliance

One-touch automated macros

Define user profiles and preferences

Generate extensive reports and export data

Multiple user network license available

More …

SPECIFICATIONS
 

MegaCam 800 Camera

Darkroom:

Light tight

Wide access door with UV safety shutoff

Gel viewing window

Sliding tray for Transilluminator

Epi white and UV illumination (365nn and 480nm)

Emission Filters - five positions:

SYBR Green 515-570nm

SYBR Gold 485-655nm

EtBr Red 570-640nm

Software:

Interface for automated controls

VisionWorksLS (system may be configured with software or optional, check with a UVP representative)

Windows 2000 SP4, Windows XP Pro SP2  

UV Transilluminator's: Select from FirstLight or Benchtop models (system may be configured with software or is optional, check with your UVP representative)

White Light Illuminator (27x27cm) with uniformity at (<5% CV)

Note:  FirstLight UV Transilluminator offers extremely uniform UV (<5% CV) which is important for capturing consistent 2D gels and quantitation

Note:  VisionWorks 2D Lite or Pro Analysis Software are order separately

OPTIONS

VisionWorks 2D Lite Analysis Software

VisionWorks 2D Pro Analysis Software

BioLite MultiSpectral Source:

Fiber Optic Epi-Illumination

Fiber Optic Transillumination

Transilluminator's: Select from the FirstLight or Benchtop models

Converter Plates:

Visi-Blue Converter Plate

Longwave Converter Plate

Thermal Printer: For archive quality prints in seconds

Gel-Tools:

Gel-Sentry

Step Tablet

Gel-Cutter

Gel-Ruler

Gel-Scooper

Gel-Trays

Computer and monitor

Flat Field Correction (FFC) Plate for Microtiter Plates

BioSpectrum 800 Imaging System
VisionWorks 2D Lite Analysis Software
VisionWorks 2D Pro Analysis Software

For specifications, refer to the Specifications tab

For optional equipment, refer to the Options tab

Single, multiple user and network licenses available.

A guided workflow for straightforward 2D analysis. Get rapid results from your proteomics data …

VisionWorks 2D Software provides fast results from 2D gels. It offers an economical solution to 2D analysis problems commonly faced by occasional or low throughput users, such as lack of reproducibility, labor intensive protocols and low confidence in results. VisionWorks 2D offers a guided approach for data analysis with the added benefits of speed and objectivity. Get the answers needed using an optimized workflow to quickly find significant proteins for subsequent picking and reporting.

Select from two different VisionWorks 2D packages:

VisionWorks 2D Lite which is limited to six same-stain gel image comparison and VisionWorks 2D Pro which has no gel image limit.

VisionWorks 2D Software enables

 

Simplicity.   A simplified workflow guides the user through each analysis step allowing effortless identification of significantly changing proteins.

Objectivity.   The guided workflow promotes a consistent analysis approach allowing new or inexperienced users to generate completely objective and reproducible results from their 2D gels.

Speed.   Analysis times of less than 10 minutes per image* are typical due to the high streamlined and optimized workflow.

* Timings based on a single stain experiment with 2 groups, 5 replicates per group and an image size of 9MB.

Unique VisionWorks 2D Approach

VisionWorks 2D is a unique and validated approach delivering a major advance for the analysis of single or multiple dyes. At the foundation of the VisionWorks 2D approach is highly accurate technology which makes pixel perfect alignment of images achievable. This produces 100% matching of spots across all images and no missing values in the analyzed data. The results are statistically robust so users have absolute confidence in the data generated.

Performing spot detection on perfectly aligned images also means that spot editing is not required on most experiments, so operator bias can be kept to an absolute minimum. The result is an incredibly rapid analysis to quickly identify the significant proteins with negligible effort.

Key Steps in the Workflow

Image Quality Control
 

Automatic image quality control assessment and feedback at the start of workflow highlights many potential issues with the image quality before proceeding to the next step

Image Alignment
 

Advanced image alignment guides users through the process of adding vector landmarks in a logical manner across the gel image

Automatic alignment process uses the landmarks set by users to fully align all experimental images

Image Alignment Review
 

Alignment grid draws attention to problematic areas on the gel image which are most distorted, so users can focus on those areas during the systematic alignment review

Alignment can be manually adjusted if required

Prefiltering and Same Spots Analysis
 

Unwanted artifacts can be filtered out of the VisionWorks 2D Analysis

Fully automatic VisionWorks 2D Analysis produces data with 100% matching and no missing values which allows robust statistical analysis

TOP 

PRICES SUBJECT TO CHANGE WITHOUT NOTICE

MINIMUM ORDER $50.00